Genetic engineering of Synechocystis sp. PCC6803 for poly-β-hydroxybutyrate overproduction

High-throughput screen for poly-3-hydroxybutyrate in Escherichia coli and Synechocystis sp. strain PCC6803.

A novel, quantitative method for detecting poly-3-hydroxybutyrate (PHB) amounts in viable cells was developed to allow for high-throughput screening of mutant libraries. The staining technique was demonstrated and optimized for the cyanobacterium Synechocystis sp. strain PCC6803 and the eubacterium Escherichia coli to maximize the fluorescence difference between PHB-accumulating and control cel...

Phycobilisome assembly in Synechocystis sp. strain PCC6803

fabricating electrospun poly(3-hydroxybutyrate)/hydroxyapatite nanocomposite scaffold for tissue engineering application

. در رســاله ی حاضر، ابتدا داربست نانوالیاف سه بعدی متخلخل به روش الگوبرداری تصویری شبیه سازی شد. با استفاده از طراحی آزمایش به روش تاگوچی پس از ساخت داربست های پلی (3- هیدروکسی بوتیرات) (p3hb) شناسایی رفتار پارامترهای موادی مثل لزجت سیال پلیمر، فراریت و رسانایی الکتریکی محلول پلیمری و پارامترهای دستگاهی مثل دبی حجمی سیال، ولتاژ الکتریکی و فاصله نازل تا جمع کننده صورت گرفت. بعد از محاسبه ی خصوص...

Exploring metabolic engineering design principles for the photosynthetic production of lactic acid by Synechocystis sp. PCC6803

BACKGROUND Molecular engineering of the intermediary physiology of cyanobacteria has become important for the sustainable production of biofuels and commodity compounds from CO2 and sunlight by "designer microbes." The chemical commodity product L-lactic acid can be synthesized in one step from a key intermediary metabolite of these organisms, pyruvate, catalyzed by a lactate dehydrogenase. Syn...

Genetic engineering of Synechocystis PCC6803 for the photoautotrophic production of the sweetener erythritol

BACKGROUND Erythritol is a polyol that is used in the food and beverage industry. Due to its non-caloric and non-cariogenic properties, the popularity of this sweetener is increasing. Large scale production of erythritol is currently based on conversion of glucose by selected fungi. In this study, we describe a biotechnological process to produce erythritol from light and CO2, using engineered ...